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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UAP1 Polyclonal antibody

UAP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16318-1-AP

Synonyms

AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UAP1 Polyclonal antibody (16318-1-AP)

    WB analysis using 16318-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16318-1-AP (UAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using UAP1 Polyclonal antibody (16318-1-AP)

    IF Staining of PC-3 using 16318-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (16318-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16318-1-AP targets UAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UAP1 fusion protein Ag9371 相同性解析による交差性が予測される生物種
Full Name UDP-N-acteylglucosamine pyrophosphorylase 1
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57-59 kDa
GenBank accession numberBC009377
Gene symbol UAP1
Gene ID (NCBI) 6675
RRIDAB_2878242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness.

Protocols

Product Specific Protocols
WB protocol for UAP1 antibody 16318-1-APDownload protocol
IF protocol for UAP1 antibody 16318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Agric Food Chem

Ginsenoside-Rb1 Improved Diabetic Cardiomyopathy through Regulating Calcium Signaling by Alleviating Protein O-GlcNAcylation.

Authors - Linhui Qin
View Article