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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UAP1L1 Polyclonal antibody

UAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25262-1-AP

Synonyms

UDP-N-acetylhexosamine pyrophosphorylase-like protein 1, EC:2.7.7.-

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of MDA-MB-453s using 25262-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of HepG2 using 25262-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of A375 using 25262-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of K-562 using 25262-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UAP1L1 Polyclonal antibody (25262-1-AP)

    IHC staining of human ovary cancer using 25262-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UAP1L1 Polyclonal antibody (25262-1-AP)

    IHC staining of human ovary cancer using 25262-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    IF Staining of HepG2 using 25262-1-AP

    Immunofluorescent analysis of HepG2 cells using 25262-1-AP (UAP1L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMDA-MB-453s cells, A375 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25262-1-AP targets UAP1L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name UDP-N-acteylglucosamine pyrophosphorylase 1-like 1
Calculated molecular weight 57 kDa
Observed molecular weight 57-59 kDa
GenBank accession numberNM_207309
Gene symbol UAP1L1
Gene ID (NCBI) 91373
RRIDAB_2879993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UAP1L1 antibody 25262-1-APDownload protocol
IHC protocol for UAP1L1 antibody 25262-1-APDownload protocol
IF protocol for UAP1L1 antibody 25262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols