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  • Featured Product
  • KD/KO Validated

UBA2 Polyclonal antibody

UBA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15347-1-AP

Synonyms

SUMO-activating enzyme subunit 2, SAE2, HRIHFB2115, EC:2.3.2.-, ARX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBA2 Polyclonal antibody (15347-1-AP)

    WB analysis using 15347-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using UBA2 Polyclonal antibody (15347-1-AP)

    WB analysis of human brain using 15347-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using UBA2 Polyclonal antibody (15347-1-AP)

    WB analysis of Jurkat using 15347-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using UBA2 Polyclonal antibody (15347-1-AP)

    WB analysis of human brain using 15347-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using UBA2 Polyclonal antibody (15347-1-AP)

    IP experiment of Jurkat using 15347-1-AP

    IP result of anti-UBA2 (IP:15347-1-AP, 4ug; Detection:15347-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBA2 Polyclonal antibody (15347-1-AP)

    IHC staining of human breast cancer using 15347-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15347-1-AP (UBA2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBA2 Polyclonal antibody (15347-1-AP)

    IF Staining of HeLa using 15347-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15347-1-AP (UBA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, human brain tissue, Jurkat cells, HeLa cells, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15347-1-AP targets UBA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBA2 fusion protein Ag7586 相同性解析による交差性が予測される生物種
Full Name ubiquitin-like modifier activating enzyme 2
Calculated molecular weight 71 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003153
Gene symbol UBA2
Gene ID (NCBI) 10054
RRIDAB_2211586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBA2 antibody 15347-1-APDownload protocol
IHC protocol for UBA2 antibody 15347-1-APDownload protocol
IF protocol for UBA2 antibody 15347-1-APDownload protocol
IP protocol for UBA2 antibody 15347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IHC

Cell Death Differ

Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.

Authors - Jing Huang
  • KD Validated
View Article