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UBA6 Polyclonal antibody

UBA6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13211-1-AP

Synonyms

MOP4, MOP 4, Monocyte protein 4, EC:6.2.1.45, E1-L2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBA6 Polyclonal antibody (13211-1-AP)

    WB analysis using 13211-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UBA6 Polyclonal antibody (13211-1-AP)

    WB analysis of HEK-293 using 13211-1-AP

    WB result of UBA6 antibody (13211-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA6 transfected HEK-293 cells.

  • Western Blot (WB) analysis of human brain tissue using UBA6 Polyclonal antibody (13211-1-AP)

    WB analysis of human brain using 13211-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBA6 Polyclonal antibody (13211-1-AP)

    IHC staining of mouse testis using 13211-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBA6 Polyclonal antibody (13211-1-AP)

    IHC staining of mouse testis using 13211-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UBA6 Polyclonal antibody (13211-1-AP)

    IHC staining of mouse brain using 13211-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UBA6 Polyclonal antibody (13211-1-AP)

    IHC staining of mouse brain using 13211-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13211-1-AP (UBA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using UBA6 Polyclonal antibody (13211-1-AP)

    IF Staining of SH-SY5Y using 13211-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 13211-1-AP (UBA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13211-1-AP targets UBA6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBA6 fusion protein Ag4000 相同性解析による交差性が予測される生物種
Full Name ubiquitin-like modifier activating enzyme 6
Calculated molecular weight 1052 aa, 118 kDa
Observed molecular weight 118 kDa
GenBank accession numberBC031637
Gene symbol UBA6
Gene ID (NCBI) 55236
RRIDAB_2211747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBA6(Ubiquitin-like modifier-activating enzyme 6) is also named as MOP4, UBE1L2 and belongs to the ubiquitin-activating E1 family. It probably acts as a single molecule like UBE1 and not as a heterodimeric E1 complex, as formed by AOS1/Uba2 and APP-BP1/Uba3. UBA6 is even more restricted in distribution because it is only found in vertebrates but not in lower organisms so that it might have a specialized role in tissues of higher organisms, and in particular, in the testis, where it is expressed by far most abundantly.(PMID:17580310). It has 4 isoforms produced by alternative splicing. This antibody recognizes the full-length(118 kda) and the truncated isoform CRA_b (60-70 kDa) of UBA6 (NCBI).

Protocols

Product Specific Protocols
WB protocol for UBA6 antibody 13211-1-APDownload protocol
IHC protocol for UBA6 antibody 13211-1-APDownload protocol
IF protocol for UBA6 antibody 13211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Nat Commun

Inhibition of UBA6 by inosine augments tumour immunogenicity and responses

Authors - Lei Zhang
View Article