Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human heart tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66993-1-Ig targets UBAP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | UBAP1 fusion protein Ag17675 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin associated protein 1 |
| Calculated molecular weight | 502 aa, 55 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC020950 |
| Gene Symbol | UBAP1 |
| Gene ID (NCBI) | 51271 |
| RRID | AB_2882310 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NZ09 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBAP1 antibody 66993-1-Ig | Download protocol |
| IHC protocol for UBAP1 antibody 66993-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |