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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBAP1 Monoclonal antibody

UBAP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6A2

Cat no : 66993-1-Ig

Synonyms

NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBAP1 Monoclonal antibody (66993-1-Ig)

    WB analysis using 66993-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66993-1-Ig (UBAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using UBAP1 Monoclonal antibody (66993-1-Ig)

    IHC staining of human heart using 66993-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using UBAP1 Monoclonal antibody (66993-1-Ig)

    IHC staining of human heart using 66993-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBAP1 Monoclonal antibody (66993-1-Ig)

    IHC staining of human lung cancer using 66993-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBAP1 Monoclonal antibody (66993-1-Ig)

    IHC staining of human lung cancer using 66993-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66993-1-Ig targets UBAP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBAP1 fusion protein Ag17675 相同性解析による交差性が予測される生物種
Full Name ubiquitin associated protein 1
Calculated molecular weight 502 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC020950
Gene symbol UBAP1
Gene ID (NCBI) 51271
RRIDAB_2882310
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

Protocols

Product Specific Protocols
WB protocol for UBAP1 antibody 66993-1-IgDownload protocol
IHC protocol for UBAP1 antibody 66993-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols