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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UBAP2 Recombinant antibody (83885-2-RR)

    WB analysis of HeLa using 83885-2-RR

    WB result of UBAP2 antibody (83885-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBAP2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using UBAP2 Recombinant antibody (83885-2-RR)

    WB analysis using 83885-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83885-2-RR (UBAP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBAP2 Recombinant antibody (83885-2-RR)

    IF Staining of HeLa using 83885-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBAP2 antibody (83885-2-RR, Clone: 240890F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of U-2 OS using UBAP2 Recombinant antibody (83885-2-RR)

    FC experiment of U-2 OS using 83885-2-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug UBAP2 Recombinant antibody (83885-2-RR, Clone:240890F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83885-2-RR

    Affinity and Kinetic Characterization of 83885-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83885-2-RR against Human UBAP2 were performed. The affinity constant is 0.59 nM.

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  • KD/KO Validated

UBAP2 Recombinant antibody

UBAP2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83885-2-RR
Clone No.240890F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

ubiquitin associated protein 2, UBAP 2, KIAA1491, bA176F3.5, 240890F6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HCT 116 cells, HEK-293T cells, A549 cells, Jurkat cells, U2OS cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83885-2-RR targets UBAP2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UBAP2 fusion protein Ag30572 相同性解析による交差性が予測される生物種
Full Name ubiquitin associated protein 2
Calculated molecular weight 117 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_018449
Gene Symbol UBAP2
Gene ID (NCBI) 55833
RRIDAB_3671467
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5T6F2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBAP2 antibody 83885-2-RRDownload protocol
IF protocol for UBAP2 antibody 83885-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols