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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

UBC Polyclonal antibody

UBC Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10457-1-AP

Synonyms

HMG20, Polyubiquitin C, UBC, ubiquitin C

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human lung cancer using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human lung cancer using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human urothelial carcinoma using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human urothelial carcinoma using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of mouse stomach using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat liver using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat pancreas using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat stomach using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of mouse liver using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of mouse liver using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat stomach using 10457-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse liver tissue, human lung cancer tissue, human urothelial carcinoma tissue, mouse stomach tissue, rat liver tissue, rat pancreas tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10457-1-AP targets UBC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBC fusion protein Ag0711 相同性解析による交差性が予測される生物種
Full Name ubiquitin C
Calculated molecular weight 60 kDa
GenBank accession numberBC008955
Gene symbol UBC
Gene ID (NCBI) 7316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin (Ub) is a small, highly conserved eukaryotic protein that has an essential role in diverse cellular signaling pathways, including targeting proteins for proteasomal degradation. Cellular Ub is comprised of two distinct pools consisting of free Ub and Ub-substrate conjugates. UbC is a polyubiquitine gene that encodes Ub polyprotein. UbC, as stress-inducible polyubiquitin precursor proteins of around 9-11 monomers, is cleaved into monomeric ubiquitin by cellular deubiquitylating enzymes.

Protocols

Product Specific Protocols
IHC protocol for UBC antibody 10457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.

Authors - Yuan Tian
  • KD Validated
View Article

Chem Biol Interact

Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells.

Authors - Zhan-Jun Ma
View Article
humanWB

Neuroscience

Maresin 1 Activates LGR6 to Alleviate Neuroinflammation via the CREB/JMJD3/IRF4 Pathway in a Rat Model of Subarachnoid Hemorrhage

Authors - Zhenyan Li
View Article