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  • Featured Product
  • KD/KO Validated

UBC12 Monoclonal antibody, PBS Only

UBC12 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H8D2

Cat no : 67482-1-PBS

Synonyms

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBC12 Monoclonal antibody (67482-1-Ig)

    WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67482-1-Ig (UBC12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UBC12 Monoclonal antibody (67482-1-Ig)

    WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

    WB result of UBC12 antibody (67482-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UBC12 Monoclonal antibody (67482-1-Ig)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UBC12 Monoclonal antibody (67482-1-Ig)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBC12 Monoclonal antibody (67482-1-Ig)

    IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC12 antibody (67482-1-Ig, Clone: 1H8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67482-1-PBS targets UBC12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBC12 fusion protein Ag5984 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC058924
Gene symbol UBC12
Gene ID (NCBI) 9040
RRIDAB_2882709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.