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  • Featured Product
  • KD/KO Validated

UBC12 Monoclonal antibody, PBS Only

UBC12 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H8D2

Cat no : 67482-1-PBS

Synonyms

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBC12 Monoclonal antibody (67482-1-Ig)

    WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67482-1-Ig (UBC12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UBC12 Monoclonal antibody (67482-1-Ig)

    WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

    WB result of UBC12 antibody (67482-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UBC12 Monoclonal antibody (67482-1-Ig)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UBC12 Monoclonal antibody (67482-1-Ig)

    IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBC12 Monoclonal antibody (67482-1-Ig)

    IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC12 antibody (67482-1-Ig, Clone: 1H8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67482-1-PBS targets UBC12 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBC12 fusion protein Ag5984 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC058924
Gene symbol UBE2M
Gene ID (NCBI) 9040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.