UBC12 Recombinant antibody
UBC12 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240477H5
Cat no : 83628-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, L02 cells, SMMC-7721 cells, A549 cells, HT-1080 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83628-1-RR is UBC12 Fusion Protein expressed in E. coli.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | UBC12 fusion protein Ag5984 相同性解析による交差性が予測される生物種 |
Full Name | ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 17-20 kDa |
GenBank accession number | BC058924 |
Gene symbol | UBC12 |
Gene ID (NCBI) | 9040 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBE2M (Ubiquitin-conjugating enzyme E2 M), also called UBC12, accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction between UBE2M and the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (PMID: 10207026; 15361859).
Protocols
Product Specific Protocols | |
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WB protocol for UBC12 antibody 83628-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |