UBC9 Polyclonal antibody
UBC9 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10224-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse spleen tissue, HEK-293 cells, human testis tissue, Jurkat cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
10224-1-AP targets UBC9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | UBC9 fusion protein Ag0252 相同性解析による交差性が予測される生物種 |
Full Name | ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) |
Calculated molecular weight | 18 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC000427 |
Gene symbol | UBC9 |
Gene ID (NCBI) | 7329 |
RRID | AB_2210593 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).
Protocols
Product Specific Protocols | |
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WB protocol for UBC9 antibody 10224-1-AP | Download protocol |
IHC protocol for UBC9 antibody 10224-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |