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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBC9 Polyclonal antibody

UBC9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10224-1-AP

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using UBC9 Polyclonal antibody (10224-1-AP)

    WB analysis of mouse spleen using 10224-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using UBC9 Polyclonal antibody (10224-1-AP)

    WB analysis of Jurkat using 10224-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using UBC9 Polyclonal antibody (10224-1-AP)

    WB analysis of mouse spleen using 10224-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using UBC9 Polyclonal antibody (10224-1-AP)

    WB analysis of human testis using 10224-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UBC9 Polyclonal antibody (10224-1-AP)

    WB analysis of HEK-293 using 10224-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using UBC9 Polyclonal antibody (10224-1-AP)

    WB analysis of Jurkat using 10224-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC9 Polyclonal antibody (10224-1-AP)

    IHC staining of human lung cancer using 10224-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC9 Polyclonal antibody (10224-1-AP)

    IHC staining of human lung cancer using 10224-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse spleen tissue, HEK-293 cells, human testis tissue, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10224-1-AP targets UBC9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBC9 fusion protein Ag0252 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Calculated molecular weight 18 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC000427
Gene symbol UBC9
Gene ID (NCBI) 7329
RRIDAB_2210593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Protocols

Product Specific Protocols
WB protocol for UBC9 antibody 10224-1-APDownload protocol
IHC protocol for UBC9 antibody 10224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols