Validation Data Gallery

Filter: AllWBIHC

WB analysis of HEK-293 using 15912-1-AP
WB result of UBE1 antibody (15912-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE1 transfected HEK-293 cells.
WB analysis of HL-60 using 15912-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15912-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15912-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 15912-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 15912-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 15912-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 15912-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 15912-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15912-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 15912-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15912-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

UBE1 Polyclonal antibody

UBE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15912-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

UBA1, GXP1, EC:6.2.1.45, AMCX1, A1ST

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, HeLa cells, HEK-293 cells, mouse spleen tissue, rat spleen tissue, mouse colon tissue
Positive IHC detected in	human colon cancer tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:200-1:1500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 4 publications below

Product Information

15912-1-AP targets UBE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBE1 fusion protein Ag8703 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-like modifier activating enzyme 1
Calculated molecular weight	1058 aa, 118 kDa
Observed molecular weight	114-118 kDa
GenBank accession number	BC013041
Gene Symbol	UBA1
Gene ID (NCBI)	7317
RRID	AB_2211462
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22314
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UBE1(Ubiquitin-activating enzyme E1) is also named as A1S9T, UBE1 and belongs to the ubiquitin-activating E1 family. It catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation and initiates the activation and conjugation of ubiquitin-like proteins. Defects in UBE1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2). UBE1 has two isoforms with the molecular mass of 118 and 114 kDa.

Protocols

Product Specific Protocols
WB protocol for UBE1 antibody 15912-1-AP	Download protocol
IHC protocol for UBE1 antibody 15912-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cancer Discov The UBA1-STUB1 axis mediates cancer immune escape and resistance to checkpoint blockade Authors - Yi Bao View Article
human	WB	Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation Authors - Xiaohua Xu View Article
mouse	IHC	ACS Nano Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver. Authors - Yakun Wu View Article
human		Cancer Biol Med CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a. Authors - Wenqi Du View Article
human	WB	Am J Cancer Res The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex. Authors - Zheng Xu View Article
human	IHC	Front Oncol Ubiquitin-Like Modifier Activating Enzyme 1 as a Novel Diagnostic and Prognostic Indicator That Correlates With Ferroptosis and the Malignant Phenotypes of Liver Cancer Cells. Authors - Yiru Shan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 15912-1-AP

WB analysis of HL-60 using 15912-1-AP

WB analysis of HeLa using 15912-1-AP

WB analysis of HEK-293 using 15912-1-AP

WB analysis of HL-60 using 15912-1-AP

WB analysis of mouse spleen using 15912-1-AP

WB analysis of rat spleen using 15912-1-AP

WB analysis of mouse colon using 15912-1-AP

IHC staining of human colon cancer using 15912-1-AP

IHC staining of human colon cancer using 15912-1-AP

IHC staining of human normal colon using 15912-1-AP

IHC staining of human colon cancer using 15912-1-AP

UBE1 Polyclonal antibody

UBE1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The UBA1-STUB1 axis mediates cancer immune escape and resistance to checkpoint blockade

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.

CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a.

The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex.

Ubiquitin-Like Modifier Activating Enzyme 1 as a Novel Diagnostic and Prognostic Indicator That Correlates With Ferroptosis and the Malignant Phenotypes of Liver Cancer Cells.