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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBE1 Monoclonal antibody

UBE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7E8

Cat no : 67198-1-Ig

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE1 Monoclonal antibody (67198-1-Ig)

    WB analysis using 67198-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UBE1 Monoclonal antibody (67198-1-Ig)

    WB analysis using 67198-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE1 Monoclonal antibody (67198-1-Ig)

    IHC staining of human colon cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE1 Monoclonal antibody (67198-1-Ig)

    IHC staining of human colon cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE1 Monoclonal antibody (67198-1-Ig)

    IHC staining of human lung cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE1 Monoclonal antibody (67198-1-Ig)

    IHC staining of human lung cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE1 Monoclonal antibody (67198-1-Ig)

    IHC staining of human colon cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE1 Monoclonal antibody (67198-1-Ig)

    IHC staining of human colon cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBE1 Monoclonal antibody (67198-1-Ig)

    IF Staining of HeLa using 67198-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE1 antibody (67198-1-Ig, Clone: 1H7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HeLa cell, HEK-293 cells, Jurkat cells, HL-60 cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67198-1-Ig targets UBE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen UBE1 fusion protein Ag8920 相同性解析による交差性が予測される生物種
Full Name ubiquitin-like modifier activating enzyme 1
Calculated molecular weight 1058 aa, 118 kDa
Observed molecular weight 114-118 kDa
GenBank accession numberBC013041
Gene symbol UBA1
Gene ID (NCBI) 7317
RRIDAB_2882491
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE1 antibody 67198-1-IgDownload protocol
IHC protocol for UBE1 antibody 67198-1-IgDownload protocol
IF protocol for UBE1 antibody 67198-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biosci Rep

Prognostic value and chemotherapy response prediction of a proliferation essential gene signature in colon cancer

Authors - Jinsheng Liu
View Article