UBE2A Polyclonal antibody
UBE2A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11080-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, COLO 320 cells, human kidney tissue, human placenta tissue |
Positive IHC detected in | human nasopharyngeal carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
Product Information
11080-1-AP targets UBE2A in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | UBE2A fusion protein Ag1567 相同性解析による交差性が予測される生物種 |
Full Name | ubiquitin-conjugating enzyme E2A (RAD6 homolog) |
Calculated molecular weight | 17 kDa |
Observed molecular weight | 17 kDa |
GenBank accession number | BC010175 |
Gene symbol | UBE2A |
Gene ID (NCBI) | 7319 |
RRID | AB_2212040 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBE2A(Ubiquitin-conjugating enzyme E2 A) serves as the cognate E2-conjugating enzyme. It plays a critical role in regulating TP53 protein levels under both normal and stress conditions. UBE2A, UBE2B regulates TP53 levels in a "yin-yang" manner through a combination of two distinct mechanisms in mammalian cells(PMID:22083959). This protein has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for UBE2A antibody 11080-1-AP | Download protocol |
IHC protocol for UBE2A antibody 11080-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Sci A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently. | ||
Mol Med Rep Yuan‑zhi‑san inhibits tau protein aggregation in an Aβ1‑40‑induced Alzheimer's disease rat model via the ubiquitin‑proteasome system. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alexandra (Verified Customer) (03-20-2024) | I was searching for a Rad6 antibody is strong enough to visualize endogenous protein. This is it. Works pretty good with endogenous Rad6 and extremely good with transfected cell lisate (for that I recomend a higher dilution, with 1000x the signal can be too strong)
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