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  • Featured Product
  • KD/KO Validated

UBE2C Monoclonal antibody, PBS Only

UBE2C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C12H8

Cat no : 66087-1-PBS

Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2C Monoclonal antibody (66087-1-Ig)

    WB analysis using 66087-1-Ig (same clone as 66087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66087-1-Ig (UBE2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBE2C Monoclonal antibody (66087-1-Ig)

    IF Staining of HeLa using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE2C antibody (66087-1-Ig, Clone: 1C12H8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66087-1-PBS targets UBE2C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBE2C fusion protein Ag19235 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2C
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC016292
Gene symbol UBE2C
Gene ID (NCBI) 11065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).