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  • Featured Product
  • KD/KO Validated

UBE2C Monoclonal antibody, PBS Only

UBE2C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C12H8

Cat no : 66087-1-PBS

Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2C Monoclonal antibody (66087-1-Ig)

    WB analysis using 66087-1-Ig (same clone as 66087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66087-1-Ig (UBE2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBE2C Monoclonal antibody (66087-1-Ig)

    IHC staining of human colon cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBE2C Monoclonal antibody (66087-1-Ig)

    IF Staining of HeLa using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE2C antibody (66087-1-Ig, Clone: 1C12H8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66087-1-PBS targets UBE2C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBE2C fusion protein Ag19235 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2C
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC016292
Gene symbol UBE2C
Gene ID (NCBI) 11065
RRIDAB_11232220
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).