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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    WB analysis using 15475-1-AP (same clone as 15475-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    WB analysis of mouse brain using 15475-1-AP (same clone as 15475-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    WB analysis of human testis using 15475-1-AP (same clone as 15475-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    IHC staining of human breast cancer using 15475-1-AP (same clone as 15475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    IHC staining of human breast cancer using 15475-1-AP (same clone as 15475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15475-1-PBS targets UBE2D1/2/3/4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2D1/2/3/4 fusion protein Ag7779 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2D 4 (putative)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC004104
Gene Symbol UBE2D4
Gene ID (NCBI) 51619
RRIDAB_2210167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2X8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.