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  • Featured Product
  • KD/KO Validated

UBE2D1/2/3/4 Polyclonal antibody, PBS Only

UBE2D1/2/3/4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15475-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    WB analysis using 15475-1-AP (same clone as 15475-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    WB analysis of mouse brain using 15475-1-AP (same clone as 15475-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    WB analysis of human testis using 15475-1-AP (same clone as 15475-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    IHC staining of human breast cancer using 15475-1-AP (same clone as 15475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBE2D1/2/3/4 Polyclonal antibody (15475-1-AP)

    IHC staining of human breast cancer using 15475-1-AP (same clone as 15475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15475-1-PBS targets UBE2D1/2/3/4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2D1/2/3/4 fusion protein Ag7779 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2D 4 (putative)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC004104
Gene symbol UBE2D4
Gene ID (NCBI) 51619
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.