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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Monoclonal antibody (68138-1-Ig)

    WB analysis using 68138-1-Ig (same clone as 68138-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Monoclonal antibody (68138-1-Ig)

    WB analysis using 68138-1-Ig (same clone as 68138-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using UBE2D1/2/3/4 Monoclonal antibody (68138-1-Ig)

    IF Staining of A431 using 68138-1-Ig (same clone as 68138-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBE2D1/2/3/4 antibody (68138-1-Ig, Clone: 1C7D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

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UBE2D1/2/3/4 Monoclonal antibody, PBS Only

UBE2D1/2/3/4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68138-1-PBS
Clone No.1C7D12

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

E2(17)KB3, UBC4/5, UBC5C, UBCH5C, UBE2D3, Ubiquitin carrier protein D3, Ubiquitin protein ligase D3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68138-1-PBS targets UBE2D1/2/3/4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBE2D1/2/3/4 fusion protein Ag2278 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
Calculated molecular weight 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC003395
Gene Symbol UBE2D3
Gene ID (NCBI) 7323
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61077
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.