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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBE2D1/2/3/4 Polyclonal antibody

UBE2D1/2/3/4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11373-1-AP

Synonyms

E2(17)KB1, SFT, Stimulator of Fe transport, UBC4/5, UBC4/5 homolog, UBC5A, UBCH5, UBCH5A, UBE2D, UBE2D1, UBE2D1/2/3/4, UBE2D2, UBE2D3, UBE2D4, Ubiquitin carrier protein D1, Ubiquitin protein ligase D1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    WB analysis using 11373-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    WB analysis of NIH/3T3 using 11373-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:500. .

  • Western Blot (WB) analysis of HeLa cells using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    WB analysis of HeLa using 11373-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    IP experiment of Jurkat using 11373-1-AP

    IP result of anti-UBE2D1/2/3/4 (IP:11373-1-AP, 4ug; Detection:11373-1-AP 1:300) with Jurkat cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    IHC staining of human prostate cancer using 11373-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, Jurkat cells, mpuse heart tissue, mouse thymus tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11373-1-AP targets UBE2D1/2/3/4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2D1/2/3/4 fusion protein Ag1932 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC015997
Gene symbol UBE2D1
Gene ID (NCBI) 7321
RRIDAB_2210129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

Protocols

Product Specific Protocols
WB protocol for UBE2D1/2/3/4 antibody 11373-1-APDownload protocol
IHC protocol for UBE2D1/2/3/4 antibody 11373-1-APDownload protocol
IP protocol for UBE2D1/2/3/4 antibody 11373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols