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- KD/KO Validated
UBE2E2 Polyclonal antibody
UBE2E2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11844-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human thyroid gland tissue, human brain tissue, mouse embryo tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
11844-1-AP targets UBE2E2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBE2E2 fusion protein Ag2509 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) |
| Calculated molecular weight | 201 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC022332 |
| Gene symbol | UBE2E2 |
| Gene ID (NCBI) | 7325 |
| RRID | AB_2210274 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2E2 antibody 11844-1-AP | Download protocol |
| IHC protocol for UBE2E2 antibody 11844-1-AP | Download protocol |
| IF protocol for UBE2E2 antibody 11844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes Dev Sequential replication-coupled destruction at G1/S ensures genome stability.
| ||
Cell Death Dis UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer
| ||
Mol Cell Biol Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex. | ||
