UBE2E3 Polyclonal antibody

Name: UBE2E3 Polyclonal antibody
Brand: Proteintech
SKU: 15488-1-AP

UBE2E3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15488-1-AP

Synonyms

UBCE4, UBCH9, UbcM2, UBE2E3, Ubiquitin carrier protein E3, Ubiquitin protein ligase E3

Validation Data Gallery

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AllWBIHC

WB analysis of Jurkat using 15488-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15488-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15488-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 15488-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15488-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15488-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 15488-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15488-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 15488-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 15488-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 15488-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, mouse kidney tissue, mouse liver tissue, Y79 cells
Positive IHC detected in	human heart tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

15488-1-AP targets UBE2E3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	rat, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBE2E3 fusion protein Ag7838 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
Calculated molecular weight	23 kDa
Observed molecular weight	23-27 kDa
GenBank accession number	BC003554
Gene symbol	UBE2E3
Gene ID (NCBI)	10477
RRID	AB_10896456
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE2E3 antibody 15488-1-AP	Download protocol
IHC protocol for UBE2E3 antibody 15488-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cells Inhibition of miR-143-3p Restores Blood-Testis Barrier Function and Ameliorates Sertoli Cell Senescence Authors - Ziyan Xiao View Article
rat	WB	Neuropathology Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. Authors - Kazuhiko Watabe View Article
mouse	WB	Int J Mol Sci miR-143-3p Promotes Ovarian Granulosa Cell Senescence and Inhibits Estradiol Synthesis by Targeting UBE2E3 and LHCGR Authors - Jingxian Deng View Article
monkey	WB	Sci Adv TRIM37 is a primate-specific E3 ligase for Huntingtin and accounts for the striatal degeneration in Huntington's disease Authors - Yiyang Qin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBE2E3 Polyclonal antibody

UBE2E3 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of Jurkat using 15488-1-AP

WB analysis of mouse liver using 15488-1-AP

WB analysis of mouse liver using 15488-1-AP

WB analysis of Y79 using 15488-1-AP

WB analysis of mouse liver using 15488-1-AP

WB analysis of mouse kidney using 15488-1-AP

IHC staining of human heart using 15488-1-AP

IHC staining of human heart using 15488-1-AP

IHC staining of human skeletal muscle using 15488-1-AP

IHC staining of human skeletal muscle using 15488-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Inhibition of miR-143-3p Restores Blood-Testis Barrier Function and Ameliorates Sertoli Cell Senescence

Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.

miR-143-3p Promotes Ovarian Granulosa Cell Senescence and Inhibits Estradiol Synthesis by Targeting UBE2E3 and LHCGR

TRIM37 is a primate-specific E3 ligase for Huntingtin and accounts for the striatal degeneration in Huntington's disease