Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, Jurkat cells, mouse ovary tissue, rat ovary tissue |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
17056-1-AP targets UBE2F in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBE2F fusion protein Ag10764 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-conjugating enzyme E2F (putative) |
| Calculated molecular weight | 185 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC010549 |
| Gene Symbol | UBE2F |
| Gene ID (NCBI) | 140739 |
| RRID | AB_2210295 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969M7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2F antibody 17056-1-AP | Download protocol |
| IHC protocol for UBE2F antibody 17056-1-AP | Download protocol |
| IF protocol for UBE2F antibody 17056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells. | ||
Nat Commun The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness. | ||
EMBO J RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis | ||
Biochem Biophys Res Commun Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells. | ||
Signal Transduct Target Ther Neddylation is a novel therapeutic target for lupus by regulating double negative T cell homeostasis | ||
Dev Cell The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells |