• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBE2H Polyclonal antibody

UBE2H Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15685-1-AP

Synonyms

UBC8, EC:2.3.2.24, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme H, E2 20K

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis using 15685-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis of HeLa using 15685-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis of HeLa using 15685-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis of mouse kidney using 15685-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis of mouse kidney using 15685-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis of mouse brain using 15685-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis of mouse kidney using 15685-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UBE2H Polyclonal antibody (15685-1-AP)

    WB analysis of HeLa using 15685-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using UBE2H Polyclonal antibody (15685-1-AP)

    IHC staining of human colon using 15685-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15685-1-AP (UBE2H antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse kidney tissue, HeLa cells, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15685-1-AP targets UBE2H in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2H fusion protein Ag8187 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
Calculated molecular weight 183 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC006277
Gene symbol UBE2H
Gene ID (NCBI) 7328
RRIDAB_10858632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE2H antibody 15685-1-APDownload protocol
IHC protocol for UBE2H antibody 15685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth

Authors - Nelson V Simwela
View Article
humanWB

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

H (Verified Customer) (09-27-2020)

My attempt to use this antibody to detect UBE2H in HEK293T failed. The band intensity was very low, and there were many non-specific bands as well. I used bis-Tris gel and transferred the proteins to PVDF membrane. Primary antibody was incubated over night at 4'C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
UBE2H Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:15685-1-AP)