Validation Data Gallery

Filter: AllWBIHC

WB analysis using 15685-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15685-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15685-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15685-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15685-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15685-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15685-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15685-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 15685-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15685-1-AP (UBE2H antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBE2H Polyclonal antibody

UBE2H Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15685-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

UBC8, EC:2.3.2.24, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme H, E2 20K

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse brain tissue, mouse kidney tissue, HeLa cells, rat brain tissue, rat kidney tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

15685-1-AP targets UBE2H in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBE2H fusion protein Ag8187 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
Calculated molecular weight	183 aa, 21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC006277
Gene Symbol	UBE2H
Gene ID (NCBI)	7328
RRID	AB_10858632
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62256
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE2H antibody 15685-1-AP	Download protocol
IHC protocol for UBE2H antibody 15685-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	bioRxiv Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth Authors - Nelson V Simwela View Article
human	WB	Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway Authors - Sang Ah Yi View Article
mouse	WB	Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth Authors - Nelson V Simwela View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15685-1-AP

WB analysis of mouse brain using 15685-1-AP

WB analysis of mouse kidney using 15685-1-AP

WB analysis of HeLa using 15685-1-AP

WB analysis of HeLa using 15685-1-AP

WB analysis of HeLa using 15685-1-AP

WB analysis of mouse kidney using 15685-1-AP

WB analysis of mouse kidney using 15685-1-AP

IHC staining of human colon using 15685-1-AP

UBE2H Polyclonal antibody

UBE2H Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth