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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBC9-Specific Monoclonal antibody

UBC9-Specific Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8A2

Cat no : 67660-1-Ig

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, UBE2I-Specific, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBC9-Specific Monoclonal antibody (67660-1-Ig)

    WB analysis using 67660-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67660-1-Ig (UBC9-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67660-1-Ig targets UBC9-Specific in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberNM_003345
Gene symbol UBC9
Gene ID (NCBI) 7329
RRIDAB_2882857
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Protocols

Product Specific Protocols
WB protocol for UBC9-Specific antibody 67660-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols