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  • Featured Product
  • KD/KO Validated

UBE2L3 Polyclonal antibody, PBS Only

UBE2L3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14415-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of NIH/3T3 using 14415-1-AP (same clone as 14415-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of mouse testis using 14415-1-AP (same clone as 14415-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of HEK-293 using 14415-1-AP (same clone as 14415-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of K-562 using 14415-1-AP (same clone as 14415-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of rat kidney using 14415-1-AP (same clone as 14415-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of A431 using 14415-1-AP (same clone as 14415-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of MCF-7 using 14415-1-AP (same clone as 14415-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

    IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14415-1-PBS targets UBE2L3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2L3 fusion protein Ag5758 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2L 3
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC053368
Gene symbol UBE2L3
Gene ID (NCBI) 7332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).