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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of HeLa using 14415-1-AP (same clone as 14415-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of NIH/3T3 using 14415-1-AP (same clone as 14415-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of mouse testis using 14415-1-AP (same clone as 14415-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of HEK-293 using 14415-1-AP (same clone as 14415-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of K-562 using 14415-1-AP (same clone as 14415-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of A431 using 14415-1-AP (same clone as 14415-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of rat kidney using 14415-1-AP (same clone as 14415-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using UBE2L3 Polyclonal antibody (14415-1-AP)

    WB analysis of MCF-7 using 14415-1-AP (same clone as 14415-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14415-1-AP (UBE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    IP experiment of mouse kidney using 14415-1-AP (same clone as 14415-1-PBS)

    IP result of anti-UBE2L3 (IP:14415-1-AP, 3ug; Detection:14415-1-AP 1:2000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBE2L3 Polyclonal antibody (14415-1-AP)

    IHC staining of human lung cancer using 14415-1-AP (same clone as 14415-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14415-1-AP (UBE2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14415-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14415-1-PBS targets UBE2L3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2L3 fusion protein Ag5758 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2L 3
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC053368
Gene Symbol UBE2L3
Gene ID (NCBI) 7332
RRIDAB_2210891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68036
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).