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KD/KO Validated

UBE2L6 Polyclonal antibody

Name: UBE2L6 Polyclonal antibody
Brand: Proteintech
SKU: 17278-1-AP

UBE2L6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17278-1-AP

Synonyms

RIG B, UBCH8, UBE2L6, Ubiquitin carrier protein L6, Ubiquitin protein ligase L6

Validation Data Gallery

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AllWBIPIF/ICC

WB analysis of Jurkat using 17278-1-AP
WB result of UBE2L6 antibody (17278-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE2L6 transfected Jurkat cells.
WB analysis of Jurkat using 17278-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17278-1-AP (UBE2L6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17278-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17278-1-AP (UBE2L6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 17278-1-AP
IP result of anti-UBE2L6 (IP:17278-1-AP, 4ug; Detection:17278-1-AP 1:1000) with Jurkat cells lysate 2000ug.
IF Staining of HeLa using 17278-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBE2L6 antibody (17278-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 17278-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells
Positive IP detected in	Jurkat cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IHC	See 1 publications below

Product Information

17278-1-AP targets UBE2L6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBE2L6 fusion protein Ag11045 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-conjugating enzyme E2L 6
Calculated molecular weight	153 aa, 18 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC032491
Gene symbol	UBE2L6
Gene ID (NCBI)	9246
RRID	AB_2211001
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE2L6 antibody 17278-1-AP	Download protocol
IF protocol for UBE2L6 antibody 17278-1-AP	Download protocol
IP protocol for UBE2L6 antibody 17278-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Genomics Proteomics Bioinformatics High VHL Expression Reverses Warburg Phenotype and Enhances Immunogenicity in Kidney Tumor Cells. Authors - Songbiao Zhu View Article
human	WB	Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells Authors - Christopher Noyes KD Validated View Article
human	IHC	Anticancer Res Association of UBE2L6 and ABCB6 Expression With Platinum Resistance in Serous Ovarian Carcinoma Authors - Mami Shibahara View Article
human	WB	Cell Death Dis Induced TRIM21 ISGylation by IFN-β enhances p62 ubiquitination to prevent its autophagosome targeting. Authors - Jie Jin View Article
human	WB	Exp Gerontol p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6 Authors - Ying Cheng View Article
human	WB	J Inflamm Res Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis Authors - Jingjing Xu View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBE2L6 Polyclonal antibody

UBE2L6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 17278-1-AP

WB analysis of Jurkat using 17278-1-AP

WB analysis of Jurkat using 17278-1-AP

IP experiment of Jurkat using 17278-1-AP

IF Staining of HeLa using 17278-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

High VHL Expression Reverses Warburg Phenotype and Enhances Immunogenicity in Kidney Tumor Cells.

The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells

Association of UBE2L6 and ABCB6 Expression With Platinum Resistance in Serous Ovarian Carcinoma

Induced TRIM21 ISGylation by IFN-β enhances p62 ubiquitination to prevent its autophagosome targeting.

p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6

Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis