Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
17278-1-AP targets UBE2L6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBE2L6 fusion protein Ag11045 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-conjugating enzyme E2L 6 |
| Calculated molecular weight | 153 aa, 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC032491 |
| Gene Symbol | UBE2L6 |
| Gene ID (NCBI) | 9246 |
| RRID | AB_2211001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14933 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2L6 antibody 17278-1-AP | Download protocol |
| IF protocol for UBE2L6 antibody 17278-1-AP | Download protocol |
| IP protocol for UBE2L6 antibody 17278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genomics Proteomics Bioinformatics High VHL Expression Reverses Warburg Phenotype and Enhances Immunogenicity in Kidney Tumor Cells. | ||
Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells
| ||
Anticancer Res Association of UBE2L6 and ABCB6 Expression With Platinum Resistance in Serous Ovarian Carcinoma | ||
Cell Death Dis Induced TRIM21 ISGylation by IFN-β enhances p62 ubiquitination to prevent its autophagosome targeting. | ||
Exp Gerontol p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6 | ||
J Inflamm Res Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis |