Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293T cells, MOLT-4 cells, PC-3 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | A2780 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human skin tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 12 publications below |
| IHC | See 6 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 3 publications below |
Product Information
14115-1-AP targets UBE2S in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBE2S fusion protein Ag5264 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-conjugating enzyme E2S |
| Calculated molecular weight | 222 aa, 24 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC066948 |
| Gene Symbol | UBE2S |
| Gene ID (NCBI) | 27338 |
| RRID | AB_2256809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2S antibody 14115-1-AP | Download protocol |
| IHC protocol for UBE2S antibody 14115-1-AP | Download protocol |
| IP protocol for UBE2S antibody 14115-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Weakened APC/C activity at mitotic exit drives cancer vulnerability to KIF18A inhibition
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Protein Cell BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.
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Int J Biol Sci UBE2S as a novel ubiquitinated regulator of p16 and β-catenin to promote bone metastasis of prostate cancer.
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Int J Biol Sci The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. | ||
J Ovarian Res UBE2S promotes the progression and Olaparib resistance of ovarian cancer through Wnt/β-catenin signaling pathway. | ||
Biochem Biophys Res Commun UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.
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