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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using UBE2S Polyclonal antibody (18219-1-AP)

    WB analysis of HL-60 using 18219-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using UBE2S Polyclonal antibody (18219-1-AP)

    WB analysis of MCF-7 using 18219-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using UBE2S Polyclonal antibody (18219-1-AP)

    WB analysis of MDA-MB-453s using 18219-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using UBE2S Polyclonal antibody (18219-1-AP)

    WB analysis of mouse ovary using 18219-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using UBE2S Polyclonal antibody (18219-1-AP)

    WB analysis of COLO 320 using 18219-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using UBE2S Polyclonal antibody (18219-1-AP)

    IHC staining of human skin cancer using 18219-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18219-1-AP (UBE2S antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UBE2S Polyclonal antibody (18219-1-AP)

    IHC staining of human ovary cancer using 18219-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18219-1-AP (UBE2S antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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UBE2S Polyclonal antibody

UBE2S Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18219-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, COLO 320 cells, MCF-7 cells, MDA-MB-453s cells, mouse ovary tissue
Positive IHC detected inhuman skin cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18219-1-AP targets UBE2S in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2S fusion protein Ag12978 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2S
Calculated molecular weight 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC004236
Gene Symbol UBE2S
Gene ID (NCBI) 27338
RRIDAB_10598771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

Protocols

Product Specific Protocols
WB protocol for UBE2S antibody 18219-1-APDownload protocol
IHC protocol for UBE2S antibody 18219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols