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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using UBE2S Monoclonal antibody (66897-1-Ig)

    WB analysis of HEK-293 using 66897-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using UBE2S Monoclonal antibody (66897-1-Ig)

    WB analysis of Jurkat using 66897-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UBE2S Monoclonal antibody (66897-1-Ig)

    WB analysis of HEK-293 using 66897-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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UBE2S Monoclonal antibody

UBE2S Monoclonal Antibody for WB, ELISA
Cat No. 66897-1-Ig
Clone No.8C9E6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Synonyms

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66897-1-Ig targets UBE2S in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen UBE2S fusion protein Ag5378 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2S
Calculated molecular weight 222 aa, 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
RRIDAB_2882226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

Protocols

Product Specific Protocols
WB protocol for UBE2S antibody 66897-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols