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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2S Recombinant antibody (81854-1-RR)

    WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBE2S Recombinant antibody (81854-1-RR)

    IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBE2S Recombinant antibody (81854-1-RR)

    IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using UBE2S Recombinant antibody (81854-1-RR)

    IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81854-1-RR

    Affinity and Kinetic Characterization of 81854-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81854-1-RR against Human UBE2S were performed. The affinity constant is below 1 pM.

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UBE2S Recombinant antibody, PBS Only

UBE2S Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81854-1-PBS
Clone No.6D18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

6D18, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81854-1-PBS targets UBE2S in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UBE2S fusion protein Ag5264 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2S
Calculated molecular weight 222 aa, 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16763
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).