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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using UBE2V1 Polyclonal antibody (10207-2-AP)

    WB analysis of human spleen using 10207-2-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using UBE2V1 Polyclonal antibody (10207-2-AP)

    WB analysis of BxPC-3 using 10207-2-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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UBE2V1 Polyclonal antibody

UBE2V1 Polyclonal Antibody for WB, ELISA
Cat No. 10207-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CIR1, CROC 1, CROC1, UBE2V, UBE2V1, UEV 1, UEV1, UEV1A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman spleen tissue, BxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10207-2-AP targets UBE2V1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2V1 fusion protein Ag0278 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2 variant 1
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC000468
Gene Symbol UBE2V1
Gene ID (NCBI) 7335
RRIDAB_2272560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13404
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBE2V1(Ubiquitin-conjugating enzyme E2 variant 1) is also named as CROC1, UBE2V, UEV1 and belongs to the ubiquitin-conjugating enzyme family. It has no ubiquitin ligase activity on its own and the UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. CROC1 transcripts in all human tissues examined, with highest levels in brain, skeletal muscle, and kidney(PMID:9305758). It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for UBE2V1 antibody 10207-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols