Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 10689-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 10689-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10689-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10689-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10689-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10689-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10689-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10689-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10689-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10689-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10689-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10689-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 10689-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10689-1-AP (MMS2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10689-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10689-1-AP (MMS2 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 10689-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10689-1-AP (MMS2 antibody at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10689-1-AP
Immunofluorescent analysis of HeLa cells using 10689-1-AP (MMS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 10689-1-AP
Immunofluorescent analysis of HEK-293 cells using 10689-1-AP (MMS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MMS2 Polyclonal antibody

MMS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10689-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UBE2V2, EDPF1, EDPF 1, EDAF-1, EDAF 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, A375 cells, HeLa cells, HL-60 cells, human brain tissue, Jurkat cells, K-562 cells, rat liver tissue
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 2 publications below

Product Information

10689-1-AP targets MMS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MMS2 fusion protein Ag1111 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-conjugating enzyme E2 variant 2
Calculated molecular weight	16 kDa
Observed molecular weight	16-20 kDa
GenBank accession number	BC007051
Gene Symbol	MMS2
Gene ID (NCBI)	7336
RRID	AB_2211656
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15819
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MMS2 antibody 10689-1-AP	Download protocol
IHC protocol for MMS2 antibody 10689-1-AP	Download protocol
IF protocol for MMS2 antibody 10689-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	IF,WB	Transl Stroke Res MicroRNA-193a-5p Rescues Ischemic Cerebral Injury by Restoring N2-Like Neutrophil Subsets Authors - Ziping Han View Article
human	WB, IF	Biochim Biophys Acta Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection. Authors - Fan Chai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 10689-1-AP

WB analysis of A375 using 10689-1-AP

WB analysis of HeLa using 10689-1-AP

WB analysis of K-562 using 10689-1-AP

WB analysis of human brain using 10689-1-AP

WB analysis of HeLa using 10689-1-AP

WB analysis of HL-60 using 10689-1-AP

WB analysis of rat liver using 10689-1-AP

WB analysis of K-562 using 10689-1-AP

WB analysis of Jurkat using 10689-1-AP

WB analysis of HEK-293 using 10689-1-AP

WB analysis of HEK-293 using 10689-1-AP

IHC staining of human colon using 10689-1-AP

IHC staining of human colon using 10689-1-AP

IHC staining of human colon using 10689-1-AP

IF Staining of HeLa using 10689-1-AP

IF Staining of HEK-293 using 10689-1-AP

MMS2 Polyclonal antibody

MMS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

MicroRNA-193a-5p Rescues Ischemic Cerebral Injury by Restoring N2-Like Neutrophil Subsets

Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.