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KD/KO Validated

UBE3A Polyclonal antibody

Name: UBE3A Polyclonal antibody
Brand: Proteintech
SKU: 10344-1-AP

UBE3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10344-1-AP

Synonyms

E6AP ubiquitin-protein ligase, E6AP ubiquitin protein ligase, E6AP, E6 AP, AS

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10344-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 10344-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10344-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10344-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10344-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10344-1-AP
IP result of anti-UBE3A (IP:10344-1-AP, 4ug; Detection:10344-1-AP 1:300) with mouse brain tissue lysate 4000ug.
IHC staining of human thyroid cancer using 10344-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 10344-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 10344-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10344-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney using 10344-1-AP (UBE3A antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse kidney using 10344-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney using 10344-1-AP (UBE3A antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 10344-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10344-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10344-1-AP
Immunofluorescent analysis of Hela cells, using UBE3A antibody 10344-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 10344-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, mouse brain tissue, mouse lung tissue, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human thyroid cancer tissue, human kidney tissue, mouse lung tissue, rat liver tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 6 publications below
IP	See 4 publications below

Product Information

10344-1-AP targets UBE3A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBE3A fusion protein Ag0346 相同性解析による交差性が予測される生物種
Full Name	ubiquitin protein ligase E3A
Calculated molecular weight	852 aa, 98 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC002582
Gene symbol	UBE3A
Gene ID (NCBI)	7337
RRID	AB_2211801
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479). It has 3 isoforms with MW of 97-101 kDa produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for UBE3A antibody 10344-1-AP	Download protocol
IHC protocol for UBE3A antibody 10344-1-AP	Download protocol
IF protocol for UBE3A antibody 10344-1-AP	Download protocol
IP protocol for UBE3A antibody 10344-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA. Authors - Justin M Wolter View Article
mouse	IF	J Mol Cell Biol Landscape of genomic imprinting and its functions in the mouse mammary gland. Authors - Haibo Xu View Article
mouse,human	WB,IHC,IF,IP	Oncogene Celastrol recruits UBE3A to recognize and degrade the DNA binding domain of steroid receptors Authors - Qilong Tan KO Validated View Article
human	WB,IF	Cell Death Discov SNORD6 promotes cervical cancer progression by accelerating E6-mediated p53 degradation Authors - Qianhui Li View Article
human	WB,IF,IP	Int J Biol Sci UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185. Authors - Zhikun Zheng KD Validated View Article
mouse	WB	Mol Neurobiol All-Trans Retinoic Acid Exerts Neuroprotective Effects in Amyotrophic Lateral Sclerosis-Like Tg (SOD1*G93A)1Gur Mice. Authors - Yu Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBE3A Polyclonal antibody

UBE3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10344-1-AP

WB analysis of mouse lung using 10344-1-AP

WB analysis of mouse brain using 10344-1-AP

WB analysis of Jurkat using 10344-1-AP

WB analysis of K-562 using 10344-1-AP

IP experiment of mouse brain using 10344-1-AP

IHC staining of human thyroid cancer using 10344-1-AP

IHC staining of mouse lung using 10344-1-AP

IHC staining of rat liver using 10344-1-AP

IHC staining of mouse kidney using 10344-1-AP

IHC staining of mouse kidney using 10344-1-AP

IHC staining of human kidney using 10344-1-AP

IHC staining of human kidney using 10344-1-AP

IF Staining of HeLa using 10344-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA.

Landscape of genomic imprinting and its functions in the mouse mammary gland.

Celastrol recruits UBE3A to recognize and degrade the DNA binding domain of steroid receptors

SNORD6 promotes cervical cancer progression by accelerating E6-mediated p53 degradation

UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185.

All-Trans Retinoic Acid Exerts Neuroprotective Effects in Amyotrophic Lateral Sclerosis-Like Tg (SOD1*G93A)1Gur Mice.