Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, mouse testis tissue, HeLa cells, K-562 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
Product Information
18148-1-AP targets UBE4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBE4B fusion protein Ag12836 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitination factor E4B (UFD2 homolog, yeast) |
| Calculated molecular weight | 1173 aa, 133 kDa |
| Observed molecular weight | 146 kDa |
| GenBank accession number | BC093696 |
| Gene Symbol | UBE4B |
| Gene ID (NCBI) | 10277 |
| RRID | AB_2241281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95155 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE4B antibody 18148-1-AP | Download protocol |
| IHC protocol for UBE4B antibody 18148-1-AP | Download protocol |
| IF protocol for UBE4B antibody 18148-1-AP | Download protocol |
| IP protocol for UBE4B antibody 18148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins. | ||
Mol Carcinog Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma. | ||
Proteomics SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells. | ||
Onco Targets Ther The Roles of Ubiquitination Factor E4B (UBE4B) in the Postoperative Prognosis of Patients with Renal Cell Carcinoma and in Renal Tumor Cells Growth and Metastasis.
| ||
Pathol Res Pract UBE4B promotes the development of lung adenocarcinoma by enhancing proliferation, migration and glycolysis via PP2A/AKT signaling. | ||
Biochim Biophys Acta Gene Regul Mech UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated NRF1 cleavage
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