Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, mouse testis tissue, HeLa cells, K-562 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
Product Information
18148-1-AP targets UBE4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBE4B fusion protein Ag12836 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitination factor E4B (UFD2 homolog, yeast) |
| Calculated molecular weight | 1173 aa, 133 kDa |
| Observed molecular weight | 146 kDa |
| GenBank accession number | BC093696 |
| Gene Symbol | UBE4B |
| Gene ID (NCBI) | 10277 |
| RRID | AB_2241281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95155 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE4B antibody 18148-1-AP | Download protocol |
| IHC protocol for UBE4B antibody 18148-1-AP | Download protocol |
| IF protocol for UBE4B antibody 18148-1-AP | Download protocol |
| IP protocol for UBE4B antibody 18148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins. | ||
Mol Carcinog Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma. | ||
Proteomics SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells. | ||
Onco Targets Ther The Roles of Ubiquitination Factor E4B (UBE4B) in the Postoperative Prognosis of Patients with Renal Cell Carcinoma and in Renal Tumor Cells Growth and Metastasis.
| ||
Pathol Res Pract UBE4B promotes the development of lung adenocarcinoma by enhancing proliferation, migration and glycolysis via PP2A/AKT signaling. | ||
Biochim Biophys Acta Gene Regul Mech UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated NRF1 cleavage
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