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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using UBF1 Polyclonal antibody (20660-1-AP)

    WB analysis of HT-1080 using 20660-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using UBF1 Polyclonal antibody (20660-1-AP)

    WB analysis of MCF-7 using 20660-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UBF1 Polyclonal antibody (20660-1-AP)

    WB analysis of HepG2 using 20660-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20660-1-AP (UBF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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UBF1 Polyclonal antibody

UBF1 Polyclonal Antibody for WB, ELISA
Cat No. 20660-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UBTF, UBF-1, UBF 1, UBF, NOR 90

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20660-1-AP targets UBF1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name upstream binding transcription factor, RNA polymerase I
Calculated molecular weight 89 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberNM_014233
Gene Symbol UBF1
Gene ID (NCBI) 7343
RRIDAB_2878718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17480
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBTF, also named as NOR-90 or UBF-1, is a 764 amino acid protein, which may form Homodimer. UBTF recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. UBTF exists two isoform with MV 89 kDa and 85 kDa. (PMID: 1940801)

Protocols

Product Specific Protocols
WB protocol for UBF1 antibody 20660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols