Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells, HUVEC cells, mouse testis tissue, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14253-1-AP targets UBL4A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBL4A fusion protein Ag5526 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-like 4A |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC043346 |
| Gene Symbol | UBL4A |
| Gene ID (NCBI) | 8266 |
| RRID | AB_2288205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11441 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IHC protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IF protocol for UBL4A antibody 14253-1-AP | Download protocol |
| IP protocol for UBL4A antibody 14253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer | ||
J Immunol UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6.
| ||
J Nutr Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs. | ||
J Inherit Metab Dis Mutations in GET4 disrupt the transmembrane domain recognition complex pathway. | ||
Pediatr Res Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets |