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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBOX5 Monoclonal antibody (68422-1-Ig)

    WB analysis using 68422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68422-1-Ig (UBOX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UBOX5 Monoclonal antibody (68422-1-Ig)

    WB analysis using 68422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68422-1-Ig (UBOX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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UBOX5 Monoclonal antibody

UBOX5 Monoclonal Antibody for WB, ELISA
Cat No. 68422-1-Ig
Clone No.2A7A4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

KIAA0860, RING finger protein 37, RNF37, U box domain containing 5, UBCE7IP5, UBOX5, UIP5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Saos-2 cells, T-47D cells, SK-BR-3 cells, A549 cells, K-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68422-1-Ig targets UBOX5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBOX5 fusion protein Ag6573 相同性解析による交差性が予測される生物種
Full Name U-box domain containing 5
Calculated molecular weight 59 kDa
Observed molecular weight60 kDa
GenBank accession numberBC000515
Gene Symbol UBOX5
Gene ID (NCBI) 22888
RRIDAB_3085137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBOX5 antibody 68422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols