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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBP1 Polyclonal antibody

UBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13856-1-AP

Synonyms

LBP 1a, LBP 1B, LBP1, LBP1A, LBP1B, Transcription factor LBP 1, UBP1, Upstream binding protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBP1 Polyclonal antibody (13856-1-AP)

    WB analysis using 13856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13856-1-AP (UBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using UBP1 Polyclonal antibody (13856-1-AP)

    WB analysis of human kidney using 13856-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13856-1-AP (UBP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBP1 Polyclonal antibody (13856-1-AP)

    IHC staining of human breast cancer using 13856-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13856-1-AP (UBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBP1 Polyclonal antibody (13856-1-AP)

    IHC staining of human breast cancer using 13856-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13856-1-AP (UBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, human kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13856-1-AP targets UBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBP1 fusion protein Ag4830 相同性解析による交差性が予測される生物種
Full Name upstream binding protein 1 (LBP-1a)
Calculated molecular weight 60 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC047235
Gene symbol UBP1
Gene ID (NCBI) 7342
RRIDAB_2304131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBP1, also known as Transcription factor LBP-1, is a 540 amino acid protein, which is expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines. UBP1 localizes in nuclear and can be induced by HIV-1 infection of lymphocytes. UBP1 functions as a transcriptional activator in a promoter context-dependent manner and modulates the placental expression of CYP11A1. UBP1 is involved in regulation of the alpha-globin gene in erythroid cells and represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.

Protocols

Product Specific Protocols
WB protocol for UBP1 antibody 13856-1-APDownload protocol
IHC protocol for UBP1 antibody 13856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols