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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    WB analysis of mouse testis using 23372-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UBQLN3 Polyclonal antibody (23372-1-AP)

    WB analysis of HepG2 using 23372-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    WB analysis of human testis using 23372-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    IHC staining of human testis using 23372-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    IHC staining of human testis using 23372-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBQLN3 Polyclonal antibody (23372-1-AP)

    IF Staining of HepG2 using 23372-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN3 antibody (23372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

UBQLN3 Polyclonal antibody

UBQLN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23372-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UBQLN 3, Ubiquilin-3, ubiquilin3, ubiquilin 3, TUP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells, human testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23372-1-AP targets UBQLN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBQLN3 fusion protein Ag19982 相同性解析による交差性が予測される生物種
Full Name ubiquilin 3
Calculated molecular weight 655 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC036743
Gene Symbol UBQLN3
Gene ID (NCBI) 50613
RRIDAB_2879268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H347
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).

Protocols

Product Specific Protocols
WB protocol for UBQLN3 antibody 23372-1-APDownload protocol
IHC protocol for UBQLN3 antibody 23372-1-APDownload protocol
IF protocol for UBQLN3 antibody 23372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols