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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBR2 Polyclonal antibody

UBR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18853-1-AP

Synonyms

N recognin 2, KIAA0349, EC:2.3.2.27, E3 ubiquitin-protein ligase UBR2, C6orf133

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using UBR2 Polyclonal antibody (18853-1-AP)

    WB analysis of BxPC-3 using 18853-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using UBR2 Polyclonal antibody (18853-1-AP)

    WB analysis of mouse heart using 18853-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using UBR2 Polyclonal antibody (18853-1-AP)

    WB analysis of mouse cerebellum using 18853-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBR2 Polyclonal antibody (18853-1-AP)

    IHC staining of human pancreas using 18853-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBR2 Polyclonal antibody (18853-1-AP)

    IHC staining of human pancreas using 18853-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using UBR2 Polyclonal antibody (18853-1-AP)

    IHC staining of human ovary tumor using 18853-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18853-1-AP (UBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using UBR2 Polyclonal antibody (18853-1-AP)

    IF Staining of U2OS using 18853-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using UBR2 antibody (18853-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBR2 Polyclonal antibody (18853-1-AP)

    IF Staining of HepG2 using 18853-1-AP

    Immunofluorescent analysis of HepG2 cells, using UBR2 antibody 18853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inBxPC-3 cells, mouse cerebellum tissue, mouse heart tissue
Positive IHC detected inhuman pancreas tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18853-1-AP targets UBR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBR2 fusion protein Ag13446 相同性解析による交差性が予測される生物種
Full Name ubiquitin protein ligase E3 component n-recognin 2
Calculated molecular weight439aa,50 kDa; 1755aa,201 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC064512
Gene symbol UBR2
Gene ID (NCBI) 23304
RRIDAB_10638437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for UBR2 antibody 18853-1-APDownload protocol
IHC protocol for UBR2 antibody 18853-1-APDownload protocol
IF protocol for UBR2 antibody 18853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biomed Sci

Interruption of the long non-coding RNA HOTAIR signaling axis ameliorates chemotherapy-induced cachexia in bladder cancer

Authors - Che-Yuan Hu
View Article