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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBR2 Polyclonal antibody, PBS Only

UBR2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18853-1-PBS

Synonyms

N recognin 2, KIAA0349, EC:2.3.2.27, E3 ubiquitin-protein ligase UBR2, C6orf133

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using UBR2 Polyclonal antibody (18853-1-AP)

    WB analysis of BxPC-3 using 18853-1-AP (same clone as 18853-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using UBR2 Polyclonal antibody (18853-1-AP)

    WB analysis of mouse heart using 18853-1-AP (same clone as 18853-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using UBR2 Polyclonal antibody (18853-1-AP)

    WB analysis of mouse cerebellum using 18853-1-AP (same clone as 18853-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBR2 Polyclonal antibody (18853-1-AP)

    IHC staining of human pancreas using 18853-1-AP (same clone as 18853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBR2 Polyclonal antibody (18853-1-AP)

    IHC staining of human pancreas using 18853-1-AP (same clone as 18853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using UBR2 Polyclonal antibody (18853-1-AP)

    IHC staining of human ovary tumor using 18853-1-AP (same clone as 18853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18853-1-AP (UBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using UBR2 Polyclonal antibody (18853-1-AP)

    IF Staining of U2OS using 18853-1-AP (same clone as 18853-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using UBR2 antibody (18853-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBR2 Polyclonal antibody (18853-1-AP)

    IF Staining of HepG2 using 18853-1-AP (same clone as 18853-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using UBR2 antibody 18853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18853-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18853-1-PBS targets UBR2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBR2 fusion protein Ag13446 相同性解析による交差性が予測される生物種
Full Name ubiquitin protein ligase E3 component n-recognin 2
Calculated molecular weight439aa,50 kDa; 1755aa,201 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC064512
Gene symbol UBR2
Gene ID (NCBI) 23304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.