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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBR2 Monoclonal antibody

UBR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D5H2

Cat no : 68565-1-Ig

Synonyms

bA49A4.1, C6orf133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, KIAA0349, N recognin 2, RP3 392M17.3, UBR2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBR2 Monoclonal antibody (68565-1-Ig)

    WB analysis using 68565-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68565-1-Ig (UBR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68565-1-Ig targets UBR2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBR2 fusion protein Ag13446 相同性解析による交差性が予測される生物種
Full Name ubiquitin protein ligase E3 component n-recognin 2
Calculated molecular weight439aa,50 kDa; 1755aa,201 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC064512
Gene symbol UBR2
Gene ID (NCBI) 23304
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for UBR2 antibody 68565-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols