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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBR4 Polyclonal antibody

UBR4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15966-1-AP

Synonyms

RBAF600, p600, N-recognin-4, EC:2.3.2.27, E3 ubiquitin-protein ligase UBR4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBR4 Polyclonal antibody (15966-1-AP)

    WB analysis using 15966-1-AP

    Various lysates were subjected to Tris-acetate gel system followed by western blot with 15966-1-AP (UBR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using UBR4 Polyclonal antibody (15966-1-AP)

    IP experiment of HeLa using 15966-1-AP

    IP result of anti-UBR4 (IP:15966-1-AP, 4ug; Detection:15966-1-AP 1:2000) with HeLa cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human pancreas cancer using 15966-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human pancreas cancer using 15966-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human lung cancer using 15966-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human lung cancer using 15966-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of mouse testis using 15966-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of mouse testis using 15966-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBR4 Polyclonal antibody (15966-1-AP)

    IF Staining of HeLa using 15966-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody (15966-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, mouse testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15966-1-AP targets UBR4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBR4 fusion protein Ag8740 相同性解析による交差性が予測される生物種
Full Name ubiquitin protein ligase E3 component n-recognin 4
Calculated molecular weight 5183 aa, 570 kDa
Observed molecular weight570 kDa, 270 kDa
GenBank accession numberBC018694
Gene symbol UBR4
Gene ID (NCBI) 23352
RRIDAB_2878202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4), also named P600, is an E3 ligase that can bind N-end rule substrates. UBR4-deficient mice die during embryogenesis and exhibit pleiotropic abnormalities (PMID: 23431188). UBR4 plays a role in membrane morphogenesis and extracellular matrix-mediated cell survival and signaling, human papillomavirus (HPV) E7-mediated cellular immortalization, and migration and development of neurons (PMID: 33658508, 25084275). The full-length UBR4 protein has a predicted size of ~570 kDa.

Protocols

Product Specific Protocols
WB protocol for UBR4 antibody 15966-1-APDownload protocol
IHC protocol for UBR4 antibody 15966-1-APDownload protocol
IF protocol for UBR4 antibody 15966-1-APDownload protocol
IP protocol for UBR4 antibody 15966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols