UBR5 Polyclonal antibody
UBR5 Polyclonal Antibody for IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 22782-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive IHC detected in | mouse testis tissue, human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
22782-1-AP targets UBR5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBR5 fusion protein Ag18753 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin protein ligase E3 component n-recognin 5 |
| Calculated molecular weight | 2799 aa, 309 kDa |
| Observed molecular weight | 309 kDa |
| GenBank accession number | BC137234 |
| Gene symbol | UBR5 |
| Gene ID (NCBI) | 51366 |
| RRID | AB_2879166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBR5 antibody 22782-1-AP | Download protocol |
| IF protocol for UBR5 antibody 22782-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors | ||
J Gastroenterol Hepatol C2orf40 inhibits hepatocellular carcinoma through interaction with UBR5. | ||
J Virol UBR5 Acts as an Antiviral Host Factor against MERS-CoV via Promoting Ubiquitination and Degradation of ORF4b | ||
Sci China Life Sci SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1 |
