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  • Featured Product
  • KD/KO Validated

UBR5 Monoclonal antibody, PBS Only

UBR5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E7A6

Cat no : 66937-1-PBS

Synonyms

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

    WB result of UBR5 antibody (66937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBR5 transfected HeLa cells. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis of PC-12 using 66937-1-Ig (same clone as 66937-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis of Neuro-2a using 66937-1-Ig (same clone as 66937-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis of HEK-293 using 66937-1-Ig (same clone as 66937-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using UBR5 Monoclonal antibody (66937-1-Ig)

    WB analysis of Jurkat using 66937-1-Ig (same clone as 66937-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using UBR5 Monoclonal antibody (66937-1-Ig)

    IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66937-1-PBS targets UBR5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBR5 fusion protein Ag18753 相同性解析による交差性が予測される生物種
Full Name ubiquitin protein ligase E3 component n-recognin 5
Calculated molecular weight 2799 aa, 309 kDa
Observed molecular weight 309 kDa
GenBank accession numberBC137234
Gene symbol UBR5
Gene ID (NCBI) 51366
RRIDAB_2882261
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.