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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBR7 Polyclonal antibody (16903-1-AP)

    WB analysis using 16903-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16903-1-AP (UBR7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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UBR7 Polyclonal antibody

UBR7 Polyclonal Antibody for WB, ELISA
Cat No. 16903-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C14orf130, N recognin 7, UBR7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16903-1-AP targets UBR7 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBR7 fusion protein Ag10445 相同性解析による交差性が予測される生物種
Full Name ubiquitin protein ligase E3 component n-recognin 7 (putative)
Calculated molecular weight 48 kDa
Observed molecular weight 48-60 kDa
GenBank accession numberBC015046
Gene Symbol UBR7
Gene ID (NCBI) 55148
RRIDAB_2256707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N806
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBR7 (C14orf130), is a 425-amino-acid putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. The N-end rule pathway is a ubiquitin-dependent system where E3 ligases called N-recognins, including UBR1, UBR2 and UBR7 (N-recognin-7), leading to target protein ubiquitination and subsequent degradation (PMID: 19008229). UBR7 contains a PHD-type zinc finger and a UBR-type zinc finger domain (PMID: 14702039), and probably undergoes acetylation (PMID: 19413330). Till date its precise function is not well known. Proteintech's UBR7 antibody 16903-1-AP is able to detect UBR7 with a theoretical MW of 48 kDa.

Protocols

Product Specific Protocols
WB protocol for UBR7 antibody 16903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols