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KD/KO Validated

UBXN1 Polyclonal antibody

Name: UBXN1 Polyclonal antibody
Brand: Proteintech
SKU: 16135-1-AP

UBXN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16135-1-AP

Synonyms

2B28, LOC51035, SAKS1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein, UBX domain protein 1, UBXD10, UBXN1

Validation Data Gallery

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AllWBIHC

WB analysis using 16135-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16135-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 16135-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 16135-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 16135-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 16135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 16135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 16135-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human liver tissue, mouse cerebellum tissue, human brain tissue, human heart tissue, HepG2 cells, Ramos cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:30000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

16135-1-AP targets UBXN1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBXN1 fusion protein Ag9146 相同性解析による交差性が予測される生物種
Full Name	UBX domain protein 1
Calculated molecular weight	33 kDa
Observed molecular weight	40-45 kDa
GenBank accession number	BC000902
Gene symbol	UBXN1
Gene ID (NCBI)	51035
RRID	AB_2210480
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UBXN1 antibody 16135-1-AP	Download protocol
IHC protocol for UBXN1 antibody 16135-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO J A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins. Authors - T Reid Alderson View Article
human	WB	Cell Rep p53 promotes antiviral innate immunity by driving hexosamine metabolism Authors - Wenjun Xia View Article
human	WB,IP	EMBO Rep UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation Authors - Brittany A Ahlstedt View Article
human	WB	Cancer Lett The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells. Authors - Kai Huang View Article
human,mouse	WB,IHC	Front Immunol PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma. Authors - Kaikai Yi View Article
human	WB	Sci Rep PMID: 29769705 Authors - Shotaro Uchida View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBXN1 Polyclonal antibody

UBXN1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 16135-1-AP

WB analysis of human liver using 16135-1-AP

WB analysis of mouse cerebellum using 16135-1-AP

WB analysis of human brain using 16135-1-AP

WB analysis of human heart using 16135-1-AP

IHC staining of mouse testis using 16135-1-AP

IHC staining of mouse testis using 16135-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins.

p53 promotes antiviral innate immunity by driving hexosamine metabolism

UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation

The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells.

PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

PMID: 29769705