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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBXN10 Polyclonal antibody

UBXN10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26062-1-AP

Synonyms

UBX domain protein 10, UBXD3, UBXN10

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using UBXN10 Polyclonal antibody (26062-1-AP)

    WB analysis of K-562 using 26062-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26062-1-AP (UBXN10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using UBXN10 Polyclonal antibody (26062-1-AP)

    WB analysis of mouse pancreas using 26062-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 26062-1-AP (UBXN10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using UBXN10 Polyclonal antibody (26062-1-AP)

    WB analysis of K-562 using 26062-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26062-1-AP (UBXN10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBXN10 Polyclonal antibody (26062-1-AP)

    IHC staining of human pancreas using 26062-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26062-1-AP (UBXN10 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBXN10 Polyclonal antibody (26062-1-AP)

    IHC staining of human pancreas using 26062-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26062-1-AP (UBXN10 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells, mouse pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26062-1-AP is UBXN10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBXN10 fusion protein Ag22037 相同性解析による交差性が予測される生物種
Full Name UBX domain protein 10
Calculated molecular weight 280 aa, 31 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC047428
Gene symbol UBXN10
Gene ID (NCBI) 127733
RRIDAB_2880358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBXN10 antibody 26062-1-APDownload protocol
IHC protocol for UBXN10 antibody 26062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Cell Sci

The p97-UBXN1 complex regulates aggresome formation.

Authors - Sirisha Mukkavalli
View Article