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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBXN2B Polyclonal antibody

UBXN2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25141-1-AP

Synonyms

UBX domain-containing protein 2B, p97 cofactor p37, NSFL1 cofactor p37

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBXN2B Polyclonal antibody (25141-1-AP)

    WB analysis using 25141-1-AP

    Neuro-2a cells lysates were subjected to SDS PAGE followed by western blot with 25141-1-AP (UBXN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UBXN2B Polyclonal antibody (25141-1-AP)

    IHC staining of human ovary cancer using 25141-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25141-1-AP (UBXN2B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using UBXN2B Polyclonal antibody (25141-1-AP)

    IF Staining of Neuro-2a using 25141-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UBXN2B antibody (25141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inNeuro-2a cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25141-1-AP targets UBXN2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBXN2B fusion protein Ag18259 相同性解析による交差性が予測される生物種
Full Name UBX domain protein 2B
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC113645
Gene symbol UBXN2B
Gene ID (NCBI) 137886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBXN2B, also called p37, is a cofactor of the p97 AAA ATPase. UBXN2B regulates spindle orientation during mitosis by limiting the levels of cortical NuMA (PMID: 29222185).

Protocols

Product Specific Protocols
WB protocol for UBXN2B antibody 25141-1-APDownload protocol
IHC protocol for UBXN2B antibody 25141-1-APDownload protocol
IF protocol for UBXN2B antibody 25141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols