Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of PC-3 using 14706-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14706-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14706-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse brain using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14706-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14706-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBXD1 antibody (14706-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 14706-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14706-1-AP (UBXD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

UBXD1 Polyclonal antibody

UBXD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14706-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UBXN6, UBXDC2, UBX domain-containing protein 6, UBX domain-containing protein 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-3 cells, HeLa cells, mouse brain tissue
Positive IHC detected in	mouse testis tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, mouse brain tissue, human spleen tissue, human lung tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 2 publications below

Product Information

14706-1-AP targets UBXD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBXD1 fusion protein Ag6394 相同性解析による交差性が予測される生物種
Full Name	UBX domain protein 6
Calculated molecular weight	50 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC008288
Gene Symbol	UBXD1
Gene ID (NCBI)	80700
RRID	AB_2210488
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZV1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for UBXD1 antibody 14706-1-AP	Download protocol
IHC protocol for UBXD1 antibody 14706-1-AP	Download protocol
IF protocol for UBXD1 antibody 14706-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Chem Biol Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Authors - Xiaojun Wu KD Validated View Article
human	IF	Acta Neuropathol Commun Proximity proteomics of C9orf72 dipeptide repeat proteins identifies molecular chaperones as modifiers of poly-GA aggregation. Authors - Feilin Liu View Article
human	WB	J Immunol UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling. Authors - Harshada Ketkar View Article
human	IF	J Cell Sci The p97-UBXN1 complex regulates aggresome formation. Authors - Sirisha Mukkavalli View Article
human	WB	Sci Rep Comprehensive analysis of endoplasmic reticulum stress related signature in head and neck squamous carcinoma Authors - Yu Miao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of PC-3 using 14706-1-AP

WB analysis of mouse brain using 14706-1-AP

WB analysis of HeLa using 14706-1-AP

IHC staining of mouse testis using 14706-1-AP

IHC staining of mouse testis using 14706-1-AP

IHC staining of human kidney using 14706-1-AP

IHC staining of human kidney using 14706-1-AP

IHC staining of human placenta using 14706-1-AP

IHC staining of human placenta using 14706-1-AP

IHC staining of human testis using 14706-1-AP

IHC staining of human testis using 14706-1-AP

IHC staining of human skin using 14706-1-AP

IHC staining of human skin using 14706-1-AP

IHC staining of human brain using 14706-1-AP

IHC staining of human brain using 14706-1-AP

IHC staining of human spleen using 14706-1-AP

IHC staining of human spleen using 14706-1-AP

IHC staining of human lung using 14706-1-AP

IHC staining of human lung using 14706-1-AP

IHC staining of human ovary using 14706-1-AP

IHC staining of human ovary using 14706-1-AP

IHC staining of mouse brain using 14706-1-AP

IHC staining of mouse brain using 14706-1-AP

IF Staining of HeLa using 14706-1-AP

IF Staining of HeLa using 14706-1-AP

UBXD1 Polyclonal antibody

UBXD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Proximity proteomics of C9orf72 dipeptide repeat proteins identifies molecular chaperones as modifiers of poly-GA aggregation.

UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling.

The p97-UBXN1 complex regulates aggresome formation.

Comprehensive analysis of endoplasmic reticulum stress related signature in head and neck squamous carcinoma