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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UCH-L1/PGP9.5 Recombinant antibody

UCH-L1/PGP9.5 Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241107B2

Cat no : 84154-4-RR

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCHL1 Recombinant antibody (84154-4-RR)

    WB analysis using 84154-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84154-4-RR (UCHL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UCH-L1/PGP9.5 Recombinant antibody (84154-4-RR)

    IHC staining of mouse brain using 84154-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-4-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using UCH-L1/PGP9.5 Recombinant antibody (84154-4-RR)

    IF Staining of mouse brain using 84154-4-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84154-4-RR

    Affinity and Kinetic Characterization of 84154-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84154-4-RR against Human UCHL1/PGP9.5 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inNeuro-2a cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84154-4-RR is UCH-L1/PGP9.5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UCH-L1/PGP9.5 fusion protein Ag6490 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight27 kDa
GenBank accession numberBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Protocols

Product Specific Protocols
WB protocol for UCH-L1/PGP9.5 antibody 84154-4-RRDownload protocol
IHC protocol for UCH-L1/PGP9.5 antibody 84154-4-RRDownload protocol
IF protocol for UCH-L1/PGP9.5 antibody 84154-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols