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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    WB analysis using 66230-1-Ig (same clone as 66230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    WB analysis of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    WB analysis of Neuro-2a using 66230-1-Ig (same clone as 66230-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    IHC staining of rat cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    IHC staining of rat cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    IF Staining of SH-SY5Y using 66230-1-Ig (same clone as 66230-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UCHL1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Y79 cells using UCH-L1/PGP9.5 Monoclonal antibody (66230-1-Ig)

    FC experiment of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

    1x10^6 Y79 cells were intracellularly stained with 0.4 ug UCHL1/PGP9.5 Monoclonal antibody (66230-1-Ig, Clone:1C9E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

UCH-L1/PGP9.5 Monoclonal antibody, PBS Only

UCH-L1/PGP9.5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66230-1-PBS
Clone No.1C9E11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66230-1-PBS targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UCH-L1/PGP9.5 fusion protein Ag6547 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
RRIDAB_2881621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09936
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)